Integration of genome wide molecular analysis and subcellular proteomics for renal cell carcinoma biomarker identification
Poster
Data di Pubblicazione:
2007
Citazione:
Integration of genome wide molecular analysis and subcellular proteomics for renal cell carcinoma biomarker identification / F. Raimondo, M. Verga, S. Ferrero, I. Cifola, R. Spinelli, L. Beltrame, C. Peano, C. Bianca, V. Angeloni, F. Rocco, F. Magni, A. Di Fronzo, V. Proserpio, M. Galli Kienle, C. Battaglia, P. Mocarelli, M. Pitto - In: Italian Proteomic Association 2. Annual National Conference : Aci Trezza(Catania), Italy, 26th-29th June 2007 : [abstracts][s.l] : ItPA, 2007. - pp. 156-156 (( Intervento presentato al 2. convegno Annual National Conference of the Italian Proteomic Association (ItPA) tenutosi a Aci Trezza (CT) nel 2007.
Abstract:
Clear cell renal carcinomas (cRCC) is representing about 3% of all kidney cancers. No biomarkers for early diagnosis of cRCC in asymptomatic patients or for post-surgery monitoring are yet available. In the context
of an italian research project aimed to the discovery of new markers for cRCC, the identification of differentially up-regulated genes associated to regions of increased DNA copy number was carried out, by genome-wide SNP copy number analysis (DNA) and transcriptomic profiling (RNA) using GeneChip
Affymetrix technology on 30 cRCC patients tissues. In order to confirm the differential expression at protein level, methods of protein enrichment were developed, preparing subcellular fractions from tumor or cortex
tissues from the same cRCC patients. We focused our study on a subset of membrane-associated proteins such as RGS1, CXCR4, CAV1, CAIX, located in the chromosome cytobands 1p31, 2p21, 7q31.1, 9p13 respectively, that were further investigated
using a subcellular comparative proteomic approach. Results obtained by SDS-PAGE and western blotting with specific antibodies show a good concordance between genomic profling, gene expression and protein
levels. Therefore, integration of DNA/RNA genome wide analysis with sucellular protein enrichment approach may provide the discovery of candidate tumour markers for cRCC.
Tipologia IRIS:
03 - Contributo in volume
Elenco autori:
F. Raimondo, M. Verga, S. Ferrero, I. Cifola, R. Spinelli, L. Beltrame, C. Peano, C. Bianca, V. Angeloni, F. Rocco, F. Magni, A. Di Fronzo, V. Proserpio, M. Galli Kienle, C. Battaglia, P. Mocarelli, M. Pitto
Link alla scheda completa:
Titolo del libro:
Italian Proteomic Association 2. Annual National Conference : Aci Trezza(Catania), Italy, 26th-29th June 2007 : [abstracts]